Recent Review

Photosensory Receptors Take Charge: Emerging Principles for Light Sensing.
Tilman Kottke, Aihua Xie, Delmar S. Larsen, & Wouter D. Hoff. 2018. Annual Review of Biophysics. 47, 291-313 Reprint


Selected Publications

Photoactive Yellow Protein

Formation of a New Buried Charge Drives a Large-amplitude Protein Quake in Photoreceptor Activation.
*Xie, A., L. Kelemen, J. Hendriks, B.J. White, K.J. Hellingwerf, & W.D. Hoff. 2001. Biochemistry 40:1510-1517 (accelerated publication).

Glu46 Donates a Proton to the 4-Hydroxy-cinnamate Anion Chromophore during the Photocycle of Photoactive Yellow Protein.
*Xie, A., W.D. Hoff, A.R. Kroon, & K.J. Hellingwerf. 1996. Biochemistry 35:14671-14678 (accelerated publication).

A Conserved Helical Capping Hydrogen Bond in PAS Domains Controls Signaling Kinetics in the Superfamily Prototype Photoactive Yellow Protein.
Kumauchi, M, S. Kaledhonkar, YX Li, M. Hara, A. Xie, WD Hoff. 2010. J. Am. Chem. Soc.132: 15320-15330. (Corresponding author)

Strong Ionic Hydrogen Bonding Causes a Spectral Isotope Effect in Photoactive Yellow Protein.
Kaledhonkar, S., M Hara, TP Stalcup, A Xie, WD Hoff. 2013. Biophys. J. 105:2577-2585.


Infrared Structural Biology

A Vibrational Spectral Marker for Probing the Hydrogen Bonding Status of Protonated Asp and Glu Residues.
Nie, B., Stutzman, J., A. Xie. 2005. Biophys. J. 88: 2833-2847.

Side-chain specific isotopic labeling of proteins for infrared structural biology: The case of ring-D-4-tyrosine isotope labeling of photoactive yellow protein.
Rathod, R, ZY Kang, SD Hartson, M Kumauchi, A Xie, WD Hoff. 2012. Protein Expression and Purification. 85: 125-132.

Structure and Stability of an Azoreductase with an FAD Cofactor from the Strict Anaerobe Clostridium perfringens.
Morrison, J.M., S. Dai, J. Ren, A. Taylor, M. Wilkerson, G. H. John, A. Xie. 2014. Protein and Peptide Letters, 21: 523-534.


Protein Dynamics

Long-lived Amide I Vibrational Modes in Myoglobin.
*Xie, A., A. van der Meer, W.D. Hoff, R.H. Austin. 2000. Phys. Rev. Lett. 84:5435-5438.

Excited State Lifetimes of Far-infrared Collective Modes in Proteins.
Xie, A., A. van der Meer, R.H. Austin. 2002. Phys. Rev. Lett. 88: 018102-1-4.

Picosecond Thermometer in the Amide I Band of Myoglobin.
Austin, R.H., A. Xie, L. van der Meer, B. Redlich, P-A Lindgard, H. Frauenfelder, D. Fu. 2005. Phys. Rev. Lett. 94:128101.

Proteins and Pressure.
*Frauenfelder, H., N.A. Alberding, A. Ansari, D. Braunstein, B.A. Cowen, M.K. Hong, I.E.T. Iben, J.B. Johnson, S. Luck, J.R. Mourant, P. Ormos, L. Reinisch, R. Scholl, A. Schulte, E. Shayamsunder, L.B. Sorensen, P.J. Steinbach, A. Xie, R.D. Young, & K.T. Yue. 1990. J. Phys. Chem. 94:1024-1037 (in alphabetic order except for the first author).

Glassy Behavior of a Protein.
*Iben, I.E.T., D. Braunstein, W. Doster, H., Frauenfelder, M.K. Hong, J.B. Johnson, S. Luck, P. Ormos, A. Schulte, P.J. Steinbach, A. Xie, & R.D. Young. 1989. Phys. Rev. Lett. 62:1916-1919. (In alphabetic order except the first author)



Flash Spectroscopy of Purple Membrane.
Xie, A.H., J.F. Nagle, & R.H. Lozier. 1987. Biophys. J. 51: 627-635.

Quantum Efficiencies of Bacteriorhodopsin Photochemical Reactions.
Xie, A. 1990. Biophys. J. 58:1127-1132.

Reversible Steps in the Bacteriorhodopsin Photocycle.
Lozier, R.H., A. Xie, J. Hofrichter, & M. Clore. 1992. Proc. Natl. Acad. Sci. USA. 89:3610-3614.

Pressure Effects on the Dark-Adaption of Bacteriorhodopsin.
Kovacs, I., G.U. Nienhaus, R. Philipp, & A. Xie. 1993. Biophysics J. 64:1187-1193.


Book Chapters

Changes in Active Site Hydrogen Bonding upon Formation of the Electronically Excited State of Photoactive Yellow Protein”.
D. Hoff, Z. Kang, M. Kumauchi1, A. Xie. Excited-State Hydrogen Bonding and Hydrogen Transfer. Edited by Ke-Li Han and Guang-Jiu Zhao. Wiley 2010